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M94A0744.TXT
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1994-10-21
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Document 0744
DOCN M94A0744
TI Optimisation of microculture methods for quantitation of HIV from plasma
and cellular fractions of peripheral blood.
DT 9412
AU Dunne A; Crowe S; Macfarlane Burnet Centre for Medical Research,
Fairfield; Hospital, Victoria.
SO Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:100 (poster no. 50).
Unique Identifier : AIDSLINE ASHM5/94348911
AB OBJECTIVE: Quantitation of HIV in plasma and PBMCs is used in clinical
trials to follow disease progression and determine drug efficacy. We
have modified the ACTG protocols to reduce cost and optimise virus
isolation. METHODS: Microculture procedures as described by the AIDS
Clinical Trials Group (ACTG) have been used as the basis for these
procedures. To date we have compared concentrations of PHA and IL-2 used
for stimulation of donor cells, number of days of stimulation of donor
cells, use of donor cells from single or multiple donors, and methods of
isolation and storage of cells and plasma from HIV-infected individuals.
RESULTS: compared to the ACTG procedures, our preliminary data suggest
that it is feasible to use stimulation medium for donor cells that has
no IL-2 and less foetal calf serum, donor cells need only to be
stimulated for as little as 30 hours before use in HIV culture, a less
expensive freezing medium for storage of HIV-infected cells provides
better recovery, and that manipulations of microcultures can be reduced
to a minimum with no effect on the outcome of the assay. CONCLUSIONS:
The ACTG protocol can be modified to be more cost effective and less
time consuming.
DE Cost-Benefit Analysis Human HIV/*ISOLATION & PURIF HIV
Infections/*MICROBIOLOGY Viremia/*MICROBIOLOGY *Virus
Cultivation/ECONOMICS MEETING ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).